Roche

Roche 454 GS Junior System

The GS Junior System brings high-throughput sequencing directly to the laboratory benchtop. Sequence over 35 million bases per instrument run and obtain sequencing read averaging 400 bases in length with GS Junior titanium series kit. The GS Junior system offers kits and protocols for whole genome shotgun, multi-span paired and, amplicon and cDNA sequencing, as well as sample multiplexing.

Grantholder

Title: Prof
First Name: Don
Last Name: Cowan
Phone: +27 21-959-2083
Email: dcowan@uwc.ac.za
Roche 454 GS Junior System

Contact Person/s

Name: Prof Don Cowan
Phone: +27 21-959-2083
Email: dcowan@uwc.ac.za

Roche 454 Genome Sequencer Junior

The Roche 454 Genome Sequencer is a next generation sequencing platform. The complete system comes with an instrument, a high performance computer, a software package for data acquisition, processing, assembling, a bead counter and a waste bottle. It is a bench top instrument. The 454 GS Junior combines emulsion polymerase chain reaction with pyrosequencing chemistry. As the DNA chain is elongated by nucleotide incorporation light is emitted. In pyrosequencing there is no need for gels dideoxynucleotide triphosphate and fluorescent dyes.

Grantholder

Title: Prof
First Name: Pascal Obong
Last Name: Bessong
Phone: +27 73-798-5920
Email: pascal.bessong@univen.ac.za
Roche 454 Genome Sequencer Junior

Contact Person/s

Name: Prof Pascal Obong Bessong
Phone: +27 15-962-8301
Email: pascal.bessong@univen.ac.za

Roche 454-GS Junior DNA sequencing platform

The 454-GS Junior DNA Sequencing Platform is focussed on small scale high-throughput DNA sequencing as opposed to the high volume sequencing capacity of the standard systems. The system produces approximately 80000 sequence reads per sequencing run at a cost reduction of approximately 75%. Each of these runs can accomodate up to 5 different samples, reducing the cost per sequence read. The low cost and flexibility of the equipment will therefore make pyrosequencing technology affordable for small and diverse research groups who are not engaged in large genome sequencing projects.

Grantholder

Title: Prof
First Name: Rosemary
Last Name: Dorrington
Phone: +27 46-622-4510
Email: r.dorrington@ru.ac.za
Roche 454-GS Junior DNA sequencing platform

Contact Person/s

Name: Professor Rosemary Dorrington
Phone: +27 46-603-8442
Email: r.dorrington@ru.ac.za

Roche xCelligence Real Time Cell Analyser (RTCA) Single Plate (SP)

The xCeLLigence Real Time Cell Analyser (RTCA) SP is a high throughput (96 samples per run), high sensitivity automated 96 microelectrode array instrument for measurement of electrical impedance in 96 well formats for the study of the mammalian cells in real time. It allows for real-time label-free analysis of whole cells to study cell number, viability, motility and cell morphology. The xCELLigence RTCA SP platform is a unique system in that as opposed to traditional end point based assays, it provides information at any point withinin an experiment time-frame.

Grantholder

Title: Prof
First Name: J
Last Name: Limson
Phone: +27 46-603-8263
Email: j.limson@ru.ac.za
Roche xCelligence Real Time Cell Analyser (RTCA) Single Plate (SP)

Contact Person/s

Name: Prof J Limson
Phone: +27 46-603-8263
Email: j.limson@ru.ac.za

Roche Lightcycler 1.5 Real-Time Thermocycler

The Lightcycler 1.5 instrument uses air for heating and cooling instead of thermal blocks to achieve high-speed thermal cycling. Ambient air is drawn into the machine by a fan and heated up by a heating coil located within the lid of the instrument. The main instrument unit contains the thermal chamber, photometer and drive units.

Contact Person/s

Name: MC le Roux
Phone: +27 12-521-5667
Email: marcelle@ul.ac.za
Roche Lightcycler 1.5 Real-Time Thermocycler

Roche B Light Cycler 2.0 Instrument

The LightCycler® 2.0 Instrument allows you to monitor amplification of 32 PCR products simultaneously, in real-time and online, with six different detection channels. The LightCycler® Software 4.1 collects fluorescence data from all six channels at each cycle and displays it immediately. This enables you to terminate PCR runs early if signal levels are adequate, or prolong them when signals are too low. Amplification occurs in specially designed reaction capillaries.

Contact Person/s

Name: Dr SH Abdul-Rasool
Phone: +27 21-959-2070
Email: sabdul-rasool@uwc.ac.za
Roche B Light Cycler 2.0 Instrument

Roche LightCycler 2.0 Real-Time PCR

The LightCycler 2.0 Instrument allows you to monitor amplification of 32 PCR products simultaneously, in real-time and online, with six different detection channels. The LightCycler Software 4.1 collects fluorescence data from all six channels at each cycle and displays it immediately. This enables you to terminate PCR runs early if signal levels are adequate, or prolong them when signals are too low. Amplification occurs in specially designed reaction capillaries.

Contact Person/s

Name: Prof H Soodyall
Phone: +27 11-489-9208
Roche LightCycler 2.0 Real-Time PCR