Illumina

Illumina MiSeq System

The Illumina MiSeq personal sequencing system is a powerful state-of-the-art “Next Generation’ (NGS) sequencer. It uses Truseq sequencing by synthesis chemistry, capable of sequencing up to 15GB high quality filtered bases per run, with up to 575 base pair read lengths. This allows assembly of small genomes or detection of target variants with unmatched accuracy, especially within homo-polymer regions. The MiSeq system workflow is easy to use, automated, and fast.

Contact Person/s

Name: Prof Maria Papathanasopolulos
Phone: +27 11-717-2618
Email: maria.papathanasopoulos@wits.ac.za

Grantholder

Title: Prof
First Name: Maria
Last Name: Papathanasopoulos
Phone: +27 11-717-2618
Email: maria.papathanasopoulos@wits.ac.za
Illumina MiSeq System

Illumina MiSeq v2 Personal Sequencing Platform

The MiSeq Sequencing Platform is a nextgeneration sequencer that integrates amplification, sequencing and data analysis in a single piece of equipment with a small footprint.

We propose to use the MiSeq to investigate the human B cell repertoire, which is vast (up to 9 million different antibodies) and therefore requires ultra-deep sequencing approaches. Our

Contact Person/s

Name: Prof Lynn Morris
Phone: +27 11-386-6362
Email: lynnm@nicd.ac.za
Illumina MiSeq v2 Personal Sequencing Platform

Illumina 1G Genome Analyser and Illumina BeadXpress

The Illumina/Solexa 1G Genome Analyser is an ultra high throughput DNA sequencer designed for cost effective sequencing of genomic and RNA transcript samples. The system utilises an innovative molecular clustering system to generate in excess of 40 million sequences per analysis using a sequencing by synthesis (SBS) technology. The current system generates some 27-35 bases of data per fragment, for a total of more than 1GB of data per run.

Contact Person/s

Name: Test
Illumina 1G Genome Analyser and Illumina BeadXpress