The MiSeq Sequencing Platform is a nextgeneration sequencer that integrates amplification, sequencing and data analysis in a single piece of equipment with a small footprint.
We propose to use the MiSeq to investigate the human B cell repertoire, which is vast (up to 9 million different antibodies) and therefore requires ultra-deep sequencing approaches. Our
aim is to investigate how HIV infection distorts the B cell compartment which will provide important clues to design an HIV vaccine. Furthermore, the NICD is a national public health institute, and several center’s have expressed interest in using this technology. These projects include bacterial strain characterization; human HLA and KIR genotyping; identifying point mutations that cause drug resistance in Neisseria gonorrhoeae, Herpes simplex virus, and typing of various STIs; and pathogen discovery.