Illumina MiSeq v2 Personal Sequencing Platform

Model: 
MiSeq v2 Personal Sequencing Platform
Make: 

The MiSeq Sequencing Platform is a nextgeneration sequencer that integrates amplification, sequencing and data analysis in a single piece of equipment with a small footprint.

We propose to use the MiSeq to investigate the human B cell repertoire, which is vast (up to 9 million different antibodies) and therefore requires ultra-deep sequencing approaches. Our

aim is to investigate how HIV infection distorts the B cell compartment which will provide important clues to design an HIV vaccine. Furthermore, the NICD is a national public health institute, and several center’s have expressed interest in using this technology. These projects include bacterial strain characterization; human HLA and KIR genotyping; identifying point mutations that cause drug resistance in Neisseria gonorrhoeae, Herpes simplex virus, and typing of various STIs; and pathogen discovery.

Status: 
Available
Condition: 
New
Category: 
Other
Disciplines: 
Microbiology, Medical virology, Genetics
Life Expectancy: 
10years
Location
Faculty: 
Virology
Department: 
Centre for HIV and STIs
1 Modderfontein Road
Sandringham
Johannesburg
2131

Contact Person/s

Name: Prof Lynn Morris
Phone: +27 11-386-6362
Email: lynnm@nicd.ac.za
Estimated Cost and Availability
Time Spent Researching: 
80
Time Spent Teaching: 
10
Time Spent Other: 
10
Funding Information
Equipment Price: 
R2,017,895
Year of Acquisition: 
2013