The PAM fluorometer is a device for measuring modulated chlorophyll-a (chl-a) fluorescence in the background of high light intensities. This is a very useful tool for the non-invasive determinations of photosynthetic electron transport rates (ETR) (i.e. an estimate of the light reaction of photosynthesis of plant and microalgal cells). The PAM fluorometer is very frequently used in combination with the LCH4 gas analyser which allows a correlation between "dark" and "light" reaction of photosynthesis. The LCH4 gas analyser is used for CO2 assimilation and transpiration rates. The PAM fluorometer is also used to study the physiology of stressed and non-stressed microalgal and plant cells in real time. This piece of equipment generates data that enables the plotting of graphs with the following parameters:
Fo - this parameter represents emission by excited chl-a molecules in the antennae structure of Photosystem II.
Fm - this parameter indicates the maximum chlorophyll fluorescence value obtained for a continuous light intensity.
Fv - this parameter indicates the variable components of the recording and relates to the maximum capacity for photochemical quenching Fv = Fm - Fo.
Fv / Fm - indicates the maximum quantum efficiency of Photosystem II and is a sensitive indicator of plant photosynthetic performance with healthy samples achieving a maximum value of Fv / Fm approximate 0.85.
TFm - this parameter indicates the time at which the maximum fluorescence value Fm is reached.
Name: Prof F Bux Phone: +27 31-373-2346
Title: Prof First Name: F Last Name: Bux Phone: +27 31-373-2346