Carl Zeiss Superresolution ELYRA S1 with SR-SIM and LSM 780 technology

Model: 
Superresolution ELYRA S1 with SR-SIM and LSM 780 technology
Manufacturer: 
Supplier: 
Carl Zeiss Superresolution ELYRA S1 with SR-SIM and LSM 780 technology

The Supperresolution ELYRA S1 utilizes SR-SIM (Super resolution Structured Illumination Microscopy) technology to yield resolution of up to double that of a conventional light microscope in both the lateral (XY = 50 - 120 nm) and the axial (Z = 150 - 350 nm) resolution which is optimal for nano research. It is compatible to standard fluorescent dyes and staining protocols. The system can acquire multi-color SR-SIM data with up to four channels. It allows the user to collect super resolved images in 3D by means of a Z-stack or optical sections. EMCCD camera technology achieves exceptional sensitivity and allows for imaging of bleach-sensitive and live specimens.

The sensitive and cooled 32 channel GaAsP detector in the LSM780 offers double the quantum efficiency than other confocal microscopes, which means that lower laser power and less fluorophore needs to be used in order to achieve results with a very high signal-to-noise ratio. This bodes well for all prolonged live cell sample work. With the LSM780 , qualitative analysis experiments such as Fluorescence Energy Transfer (FRET), Fluorescence Correlative Spectroscopy (FCS) and co-localization can easily be performed with the option to add FLIM with a separate detector at a later stage.

Both these technologies will be combined in a single platform, namely the fully motorized inverted Axio Observer with T-PMT for transmitted light.

Condition: 
New
Category: 
Microscope
Disciplines: 
Biochemistry, Biotechnology, Genetics, Medical Biochemistry, Microbiology, Polymers
Life Expectancy: 
10years
Location
Department: 
Physiological Sciences
Building: 
Mike de Vries Building
Stellenbosch University
Stellenbosch Central
Stellenbosch
7600

Contact Person/s

Name: Dr B Loos
Phone: +27 21-808-9196
Email: bloos@sun.ac.za

Grantholder

Title: Prof
First Name: LF
Last Name: Klumperman
Funding Information
Equipment Price: 
R7,870,706
Year of Acquisition: 
2012