Carl Zeiss Libra 120 PLUS Transmission Electron Microscope

The Zeiss Libra 120 Plus is a 120 kV transmission electron microscope particularly suitable for viewing biological samples. It contains an OMEGA energy filter which allows the selection of distinct electron energies for specimen viewing and analysis as a standard in-column feature. With the OMEGA filter, the operator is able to scan and select scattered electrons with energies that provide optimal resolution/contrast of regions of interest in the specimen (a process known as "contrast tuning"). This feature enables the viewing of unstained specimens, enhances structural resolution in thick sections by eliminating chromatic aberration and can potentially eliminate cutting artefacts caused by sample sectioning. The L120 is fitted with a unique Zeiss Köhler illumination system of which the principle feature is that it provides truly parallel electron beam incidence on the sample which eliminates the image distortion caused by oblique illumination in conventional systems. The instrument includes an Olympus MegaView G2 cooled CCD camera for digital viewing and image capturing. The resolution of the camera is 1376 x 1032 pixels with 14-bit dynamic range (16384 grey values). The pixel size of the CCD chip is 6.45 x 6.45 µm2 . It offers exposure times ranging from 100 µs to 160 seconds and frame rates up to 15 images per seconds at full resolution. Also included in WinTem instrument control software and iTEM image acquisition, processing and archiving software.