ApoTome Axio Z1 imager microscope

The ApoTome Axioimager Z1 is a 3-D imaging system used for contrast enhancement in fluorescence microscopy based on innovative grid projection techniques using an HBO light source. This technique permits optical sections to be analysed quickly and with very good quality. It also improves clarity and performance. The ApoTome allows discriminated images of fluorescent specimens to be produced. When compared to conventional epiflourescence methods, these optical sections feature increased contrast and enhanced optical resolution in axial direction. Due to interfering fluorescent signals originating from planes above or below the actual focal plane, optical sections are prerequisite for three-dimensional reconstruction. The ApoTome system consists of a slider, which is inserted in the plane of the luminous-field diaphragm of the incident-light path of rays, and an accompanying control box. The ApoTome imaging system comprises the following components: The Axioimager Z1 microscope, Axiovision 4 module ApoTome, High resolution microscopy camera axiocam HRM REV.2 (ESI), PC XEON with at least 512 MB RAM acc. to Carl Zeiss specifications plus monitor, ApoTome slider with ApoTome control box and accompanying transmission grids, AxioVision 4.x control software.