Photophysics

Photophysics Stopped Flow

Stopped-flow is a spectroscopic technique used for studying fast reaction mechanisms in solution over timescales of about 1ms up to 100’s seconds. In general, two reagents are rapidly mixed together and then ‘stopped’ in an observation cell. The sample cell is irradiated with (usually) monochromatic light and as the reaction proceeds the change in the recorded signal, usually a fluorescence signal or the absorbance at a specific wavelength, is recorded as a function of time.

Photophysics Stopped Flow